4QYD
Crystal Structure of the human BRPF1 bromodomain in complex with a histone H4K12ac peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-24 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 75.100, 75.100, 86.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.430 - 1.940 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d9e |
RMSD bond length | 0.007 |
RMSD bond angle | 0.950 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1402)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.430 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.139 | 0.279 |
Number of reflections | 18780 | |
<I/σ(I)> | 25.84 | 14.66 |
Completeness [%] | 99.9 | 96.9 |
Redundancy | 27.7 | 27.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 277 | 0.1 M HEPES, pH 7.5, 10% w/v polyethylene glycol (PEG) 6,000, 5% v/v (+/-)-2-methyl-2,4-pentanediol (MPD), VAPOR DIFFUSION, HANGING DROP, temperature 277K |