4QXZ
Crystal structure of a hypothetical protein from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.391, 90.376, 110.637 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.230 - 2.490 |
R-factor | 0.21212 |
Rwork | 0.210 |
R-free | 0.24918 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.337 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.490 | 2.500 |
Number of reflections | 12970 | |
Completeness [%] | 99.1 | 97.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES at pH 7.5, 25% (w/v) PEG 3350, 0.2M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |