4QXW
Crystal structure of the human CEACAM1 membrane distal amino terminal (N)-domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-23 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.07810 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 107.160, 107.160, 61.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.923 - 2.040 |
R-factor | 0.2059 |
Rwork | 0.204 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.097 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.26) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.923 | 2.100 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.198 | 0.010 |
Total number of observations | 16250 | |
Number of reflections | 23379 | |
<I/σ(I)> | 8.8 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.4 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 50 mM TrisHCL buffer 7.5, 60% Tascimate 8.0, 1% Beta-octyl glucoside, 12% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |