4QXF
crystal structure of human LGR4 and Rspo1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97925 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.867, 137.913, 82.560 |
Unit cell angles | 90.00, 100.13, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.250 |
R-factor | 0.21474 |
Rwork | 0.213 |
R-free | 0.24268 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qxe |
RMSD bond length | 0.009 |
RMSD bond angle | 1.267 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 4.800 | 2.250 |
Rmerge | 0.090 | ||
Number of reflections | 43609 | ||
Completeness [%] | 98.5 | 96.1 | 98.3 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1M MES pH 6.5, 0.2M sodium thiocyanate, 22% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |