4QW2

FMRP N-terminal domain (R138Q)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.94390
Spacegroup name	P 41 21 2
Unit cell lengths	89.087, 89.087, 121.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.901 - 2.989
R-factor	0.2293
Rwork	0.227
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o8v
RMSD bond length	0.003
RMSD bond angle	0.651
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.901	24.902	3.289
High resolution limit [Å]	2.989	4.736	2.989
Rmerge	0.147		0.710
Number of reflections	10425
<I/σ(I)>	12		2.975
Completeness [%]	99.8	100	100
Redundancy	6.3		6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	25-30% PEG5000 MME, 0.2 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 5 mM CH3Pb, VAPOR DIFFUSION, SITTING DROP, temperature 289K