4QVF
Crystal structure of Bcl-xL in complex with BIM BH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.540, 47.950, 34.180 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 31.847 - 1.531 |
| R-factor | 0.1668 |
| Rwork | 0.164 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ehr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.878 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.850 | 1.610 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.070 | 0.580 |
| Number of reflections | 24769 | |
| <I/σ(I)> | 11.9 | 2.5 |
| Completeness [%] | 97.5 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 17.5% PEG 8000, 0.2M NaCl, 0.1M phosphate citrate pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
