4QTR
Computational design of co-assembling protein-DNA nanowires
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-23 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 90.098, 90.098, 158.923 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.350 - 3.200 |
R-factor | 0.27 |
Rwork | 0.264 |
R-free | 0.32200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hhd |
RMSD bond length | 0.012 |
RMSD bond angle | 1.320 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 3.020 |
High resolution limit [Å] | 3.100 | 2.860 |
Number of reflections | 15510 | |
<I/σ(I)> | 102.5 | |
Completeness [%] | 88.5 | |
Redundancy | 10.7 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | 800 mM, NaCl, 0.2 M Potassium thiocyanate, 20% w/v polyethylene glycerol 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |