4QT2
Crystal Structure of the FK506-Binding Domain of Plasmodium Falciparum FKBP35 in complex with Rapamycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.812, 48.700, 58.933 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.540 - 1.440 |
R-factor | 0.1531 |
Rwork | 0.150 |
R-free | 0.21560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vn1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.813 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.540 |
High resolution limit [Å] | 1.440 |
Number of reflections | 19370 |
Completeness [%] | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1M MIB, 25% PEG1500, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |