4QQT
Crystal Structure of FGF Receptor (FGFR) 4 Tyrosine Kinase Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97877 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.384, 61.336, 61.084 |
Unit cell angles | 90.00, 99.01, 90.00 |
Refinement procedure
Resolution | 21.506 - 1.501 |
R-factor | 0.2227 |
Rwork | 0.222 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2psq |
RMSD bond length | 0.005 |
RMSD bond angle | 1.042 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.500 |
Rmerge | 0.058 |
Number of reflections | 49375 |
<I/σ(I)> | 53.6 |
Completeness [%] | 100.0 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.1 M MES, 20% (w/v) PEG 4000, 0.2 M Li2SO4 and 0.01 M Taurine, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |