4QQ4

CW-type zinc finger of MORC3 in complex with the amino terminus of histone H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791521
Spacegroup name	P 21 21 2
Unit cell lengths	62.622, 64.860, 36.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.050 - 1.750
R-factor	0.1828
Rwork	0.180
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o62
RMSD bond length	0.014
RMSD bond angle	1.603
Data reduction software	XDS
Data scaling software	Aimless (0.3.6)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.050	45.050	1.780
High resolution limit [Å]	1.750	9.090	1.750
Rmerge	0.123	0.047	0.958
Total number of observations		692	5929
Number of reflections	15427
<I/σ(I)>	12.5	28.4	2.3
Completeness [%]	100.0	98.2	99.9
Redundancy	6.9	4.9	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG-3350, 0.2 M ammonium chloride, vapor diffusion, sitting drop, temperature 293K