4QOZ
Crystal structure of the histone mRNA stem-loop, stem-loop binding protein (phosphorylated), and 3'hExo ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.107, 91.505, 128.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.169 - 2.304 |
R-factor | 0.1957 |
Rwork | 0.193 |
R-free | 0.24510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.120 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.169 | 2.380 |
High resolution limit [Å] | 2.304 | 2.304 |
Rmerge | 0.092 | 0.480 |
Number of reflections | 40611 | |
<I/σ(I)> | 9.5 | 1.8 |
Completeness [%] | 93.2 | 84.5 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 8% w/v Tacsimate, pH 6.0, 18% w/v PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |