4QOT
Crystal structure of human copper chaperone bound to the platinum ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-02-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.071564 |
| Spacegroup name | P 65 |
| Unit cell lengths | 78.055, 78.055, 54.628 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.490 - 2.200 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fee |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.063 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | ILMILIONE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.300 |
| High resolution limit [Å] | 1.500 | 2.200 |
| Number of reflections | 9697 | |
| Completeness [%] | 99.5 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 12mg/mlATOX-Pt(DACH)(H2O)(SO4), 65% sat Li2SO4, 100mM MES, 60mM NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
