4QOB

Crystal structure of cPOP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	110
Detector technology	CCD
Collection date	2013-11-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 3
Unit cell lengths	94.458, 94.458, 94.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.590 - 2.700
R-factor	0.25244
Rwork	0.247
R-free	0.29986
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.675
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.780
High resolution limit [Å]	2.700	2.700
Number of reflections	10004
Completeness [%]		95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	297	3.6M sodium formate, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K