4QNS
Crystal structure of bromodomain from Plasmodium faciparum GCN5, PF3D7_0823300
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .97623 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.217, 98.871, 50.439 |
| Unit cell angles | 90.00, 109.63, 90.00 |
Refinement procedure
| Resolution | 28.999 - 1.497 |
| R-factor | 0.1578 |
| Rwork | 0.156 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.050 |
| Data scaling software | Aimless (0.3.5) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.000 | 29.000 | 1.520 |
| High resolution limit [Å] | 1.500 | 8.200 | 1.500 |
| Rmerge | 0.089 | 0.062 | 0.487 |
| Total number of observations | 2966 | 21749 | |
| Number of reflections | 74224 | ||
| <I/σ(I)> | 13.9 | 32.5 | 3.9 |
| Completeness [%] | 99.6 | 97.5 | 95.8 |
| Redundancy | 6.6 | 6.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 2.0 M Ammonium sulfate 0.1 M sodium acetate pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
