4QNK
The structure of wt A. thaliana IGPD2 in complex with Mn2+ and phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9507 |
Spacegroup name | I 2 3 |
Unit cell lengths | 225.130, 225.130, 225.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.980 - 1.750 |
R-factor | 0.13154 |
Rwork | 0.129 |
R-free | 0.17949 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.532 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.190 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.118 | 0.579 |
Number of reflections | 187811 | |
<I/σ(I)> | 11.7 | 2.2 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 290 | 0.1 M Bicine, pH 9.0, 10% w/v PEG6000, VAPOR DIFFUSION, SITTING DROP, temperature 290K |