4QN9
Structure of human NAPE-PLD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-13 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 95.099, 95.099, 444.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 82.358 - 2.652 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.25332 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 2.387 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CCP4 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.358 | 2.720 |
High resolution limit [Å] | 2.652 | 2.652 |
Rmerge | 0.050 | 0.550 |
Number of reflections | 35841 | |
<I/σ(I)> | 26.7 | 3.8 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 14.1 | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.9 M lithium sulfate, 0.1 M HEPES, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |