4QM7
Structure of bacterial polynucleotide kinase bound to GTP and pDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 130 |
Detector technology | PIXEL |
Collection date | 2013-05-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.170, 67.210, 118.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.085 - 1.800 |
R-factor | 0.1649 |
Rwork | 0.163 |
R-free | 0.18820 |
Structure solution method | molecular refinement |
RMSD bond length | 0.014 |
RMSD bond angle | 1.526 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.100 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 34969 | |
Completeness [%] | 99.7 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100 mM sodium citrate, 100 mM magnesium chloride, 18-24% PEG 6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |