4QM6

Structure of bacterial polynucleotide kinase bound to GTP and RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	130
Detector technology	PIXEL
Collection date	2013-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	52.310, 74.420, 119.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.375 - 1.500
R-factor	0.1548
Rwork	0.153
R-free	0.17500
Structure solution method	molecular refinement
RMSD bond length	0.007
RMSD bond angle	1.195
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.900	1.580
High resolution limit [Å]	1.500	1.500
Number of reflections	75553
Completeness [%]	100.0	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	100 mM sodium citrate, 100 mM magnesium chloride, 18-24% PEG 6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K