4QL3
Crystal Structure of a GDP-bound G12R Oncogenic Mutant of Human GTPase KRas
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97924 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.125, 40.908, 91.806 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.504 - 1.041 |
R-factor | 0.139 |
Rwork | 0.138 |
R-free | 0.16210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.452 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.060 |
High resolution limit [Å] | 1.040 | 2.820 | 1.040 |
Rmerge | 0.068 | 0.030 | 0.558 |
Number of reflections | 68976 | ||
<I/σ(I)> | 10 | 1.93 | |
Completeness [%] | 96.6 | 95.1 | 81.8 |
Redundancy | 4.6 | 4.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M Sodium Acetate pH4.5, 0.1M Tris pH 8.5, 28% PEG 3,350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |