4QKJ
Glycosylated form of human LLT1, a ligand for NKR-P1, in this structure forming hexamers
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 70.060, 70.060, 101.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.000 - 2.750 |
R-factor | 0.23022 |
Rwork | 0.221 |
R-free | 0.29780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hup |
RMSD bond length | 0.018 |
RMSD bond angle | 1.967 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 2.760 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.093 | 0.757 |
Number of reflections | 4431 | |
<I/σ(I)> | 16.2 | 2 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 288 | 40%(v/v) PEG300 and 0.1M citrate phosphate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 288K |