4QKH
Dimeric form of human LLT1, a ligand for NKR-P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.870, 57.820, 82.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.300 - 1.800 |
| R-factor | 0.18041 |
| Rwork | 0.175 |
| R-free | 0.24992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hup |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.702 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.093 | 0.462 |
| Number of reflections | 21104 | |
| <I/σ(I)> | 11.3 | 3 |
| Completeness [%] | 91.8 | 81 |
| Redundancy | 8.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 30%(w/v) polyethylene glycol 6000, 0.1M HEPES, pH 7.0 , VAPOR DIFFUSION, SITTING DROP, temperature 294K |
