4QKF
Crystal structure of human ALKBH7 in complex with N-oxalylglycine and Mn(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 133 |
| Detector technology | CCD |
| Collection date | 2013-08-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.364, 81.958, 66.082 |
| Unit cell angles | 90.00, 119.94, 90.00 |
Refinement procedure
| Resolution | 33.370 - 1.990 |
| R-factor | 0.15925 |
| Rwork | 0.159 |
| R-free | 0.16516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qkb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Number of reflections | 40730 | |
| Completeness [%] | 96.7 | 80.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 277 | PEG pH 6 , EVAPORATION, temperature 277K |
