4QJV
The X-ray crystal structure of Rpo3/Rpo11 heterodimer of euryarchaeal RNA polymerase from Thermococcus kodakarensis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9785, 0.9788, 0.9600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.598, 88.169, 102.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.084 - 1.601 |
R-factor | 0.1799 |
Rwork | 0.179 |
R-free | 0.21080 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.095 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | PHENIX ((phenix.refine: dev_1626)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 91780 | |
<I/σ(I)> | 3.6 | |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch crystallization | 10 | 277 | 0.1M CAPS (pH 10), 0.1M ammonium dihydrogen phosphate, 34%(w/v) PEG4000, Microbatch crystallization, temperature 277K |