4QJ4
Structure of a fragment of human phospholipase C-beta3 delta472-569, bound to IP3 and in complex with Galphaq
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-04-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.923, 89.191, 92.639 |
| Unit cell angles | 90.00, 101.70, 90.00 |
Refinement procedure
| Resolution | 29.400 - 3.300 |
| R-factor | 0.21063 |
| Rwork | 0.208 |
| R-free | 0.26665 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3OHM |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.929 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.8.0049) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.221 | 0.480 |
| Number of reflections | 21289 | |
| <I/σ(I)> | 8.71 | 3.27 |
| Completeness [%] | 85.6 | 64.6 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 100 mM BisTris, 200 mM NaCl, 8% (v/v) PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
