4QJ4
Structure of a fragment of human phospholipase C-beta3 delta472-569, bound to IP3 and in complex with Galphaq
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-04-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 201.923, 89.191, 92.639 |
Unit cell angles | 90.00, 101.70, 90.00 |
Refinement procedure
Resolution | 29.400 - 3.300 |
R-factor | 0.21063 |
Rwork | 0.208 |
R-free | 0.26665 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3OHM |
RMSD bond length | 0.005 |
RMSD bond angle | 0.929 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.8.0049) |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.221 | 0.480 |
Number of reflections | 21289 | |
<I/σ(I)> | 8.71 | 3.27 |
Completeness [%] | 85.6 | 64.6 |
Redundancy | 3.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 100 mM BisTris, 200 mM NaCl, 8% (v/v) PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |