4QJ3
Structure of a fragment of human phospholipase C-beta3 delta472-559, in complex with Galphaq
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-03-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 200.623, 88.761, 92.232 |
| Unit cell angles | 90.00, 101.39, 90.00 |
Refinement procedure
| Resolution | 29.710 - 3.000 |
| R-factor | 0.19741 |
| Rwork | 0.195 |
| R-free | 0.24027 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3OHM |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.887 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.8.0049) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.527 |
| Number of reflections | 31965 | |
| <I/σ(I)> | 14.26 | 1.93 |
| Completeness [%] | 99.8 | 97.2 |
| Redundancy | 5.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277.15 | 100 mM BisTris, 200 mM NaCl, 5% PEG 3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
