4QH5
The GLIC pentameric Ligand-Gated Ion Channel (wild-type) crystallized in phosphate buffer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2011-10-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.878, 134.423, 160.003 |
| Unit cell angles | 90.00, 102.72, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.1995 |
| Rwork | 0.199 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.930 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 71886 | |
| Completeness [%] | 96.1 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 400 mM Sodium Isothiocyanate, 100 mM Sodium acetate, 16% glycerol, 12-15% PEG4000, 2% DMSO, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
