4QF8
Crystal Structure of the Complex of Phospholipase A2 with Spermidine at 1.65 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-02-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 43 |
Unit cell lengths | 51.555, 51.555, 47.417 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.780 - 1.650 |
R-factor | 0.19002 |
Rwork | 0.188 |
R-free | 0.23728 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fb2 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.781 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.087 | 0.463 |
Number of reflections | 14473 | |
<I/σ(I)> | 19.8 | 2 |
Completeness [%] | 96.7 | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Ammonium sulphate, PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |