4QEU
Crystal structure of BRD2(BD2) mutant in free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-04 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.506, 35.211, 48.489 |
Unit cell angles | 90.00, 118.82, 90.00 |
Refinement procedure
Resolution | 38.000 - 1.500 |
R-factor | 0.16399 |
Rwork | 0.163 |
R-free | 0.18862 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dvv |
RMSD bond length | 0.014 |
RMSD bond angle | 1.513 |
Data reduction software | PROTEUM PLUS (PLUS) |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.000 | 38.000 | 1.520 |
High resolution limit [Å] | 1.500 | 6.260 | 1.500 |
Rmerge | 0.062 | 0.046 | 0.440 |
Number of reflections | 18816 | ||
<I/σ(I)> | 16.1 | 47.57 | 2.47 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 5.6 | 10.17 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M sodium citrate, 0.2M ammonium sulfate, 30% PEG4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |