4QEU
Crystal structure of BRD2(BD2) mutant in free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-04 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.506, 35.211, 48.489 |
| Unit cell angles | 90.00, 118.82, 90.00 |
Refinement procedure
| Resolution | 38.000 - 1.500 |
| R-factor | 0.16399 |
| Rwork | 0.163 |
| R-free | 0.18862 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dvv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.513 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.000 | 38.000 | 1.520 |
| High resolution limit [Å] | 1.500 | 6.260 | 1.500 |
| Rmerge | 0.062 | 0.046 | 0.440 |
| Number of reflections | 18816 | ||
| <I/σ(I)> | 16.1 | 47.57 | 2.47 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 5.6 | 10.17 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M sodium citrate, 0.2M ammonium sulfate, 30% PEG4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
