4QCB
Protein-DNA complex of Vaccinia virus D4 with double-stranded non-specific DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.389, 92.327, 142.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.500 - 2.890 |
R-factor | 0.21826 |
Rwork | 0.216 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dof CHAIN A |
RMSD bond length | 0.007 |
RMSD bond angle | 1.185 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.500 | 3.050 |
High resolution limit [Å] | 2.890 | 2.890 |
Rmerge | 0.158 | 0.505 |
Number of reflections | 11991 | |
<I/σ(I)> | 6.1 | 2.3 |
Completeness [%] | 97.9 | 99.3 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2:1 protein solution/reservoir (16% PEG6000, 0.08 M Tris, pH 8.5, 20% glycerol), VAPOR DIFFUSION, HANGING DROP, temperature 293K |