4QAM

Crystal Structure of the RPGR RCC1-like domain in complex with the RPGR-interacting domain of RPGRIP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97948
Spacegroup name	P 21 21 21
Unit cell lengths	63.120, 63.800, 161.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.230 - 1.830
R-factor	0.1637
Rwork	0.162
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jhn
RMSD bond length	0.023
RMSD bond angle	2.235
Data scaling software	XSCALE
Phasing software	PHENIX (AutoSol)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.232	43.232	1.880
High resolution limit [Å]	1.830	8.180	1.830
Rmerge	0.090	0.038	0.904
Number of reflections	58347	753	4234
<I/σ(I)>	20.13	61.44	3.21
Completeness [%]	100.0	99.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% PEG3350, 300 mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K