4QAM
Crystal Structure of the RPGR RCC1-like domain in complex with the RPGR-interacting domain of RPGRIP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97948 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.120, 63.800, 161.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.230 - 1.830 |
R-factor | 0.1637 |
Rwork | 0.162 |
R-free | 0.19650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jhn |
RMSD bond length | 0.023 |
RMSD bond angle | 2.235 |
Data scaling software | XSCALE |
Phasing software | PHENIX (AutoSol) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.232 | 43.232 | 1.880 |
High resolution limit [Å] | 1.830 | 8.180 | 1.830 |
Rmerge | 0.090 | 0.038 | 0.904 |
Number of reflections | 58347 | 753 | 4234 |
<I/σ(I)> | 20.13 | 61.44 | 3.21 |
Completeness [%] | 100.0 | 99.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG3350, 300 mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |