4Q8B
The crystal structure of CotA laccase complexed with sinapic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.278, 116.612, 81.099 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 29.810 - 1.910 |
| R-factor | 0.14164 |
| Rwork | 0.139 |
| R-free | 0.18581 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gsk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.637 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.810 |
| High resolution limit [Å] | 1.910 |
| Number of reflections | 71222 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 12-15.5% PEG3350, 0.1M MgCl2, 0.1M pH 6.5 Bis-tris, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
