4Q7X
Neutrophil serine protease 4 (PRSS57) apo form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977408 |
| Spacegroup name | P 41 |
| Unit cell lengths | 70.366, 70.366, 150.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.760 - 2.550 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hne |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.550 |
| Number of reflections | 23565 |
| <I/σ(I)> | 14.1 |
| Completeness [%] | 99.2 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 292 | 16% PEG 10,000, 0.1M sodium/ammonium acetate, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
