4Q6M

Structural analysis of the apo-form of Helicobacter pylori Csd4, a D,L-carboxypeptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-13
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0722
Spacegroup name	P 21 21 21
Unit cell lengths	53.141, 66.549, 144.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.810 - 1.600
R-factor	0.19491
Rwork	0.194
R-free	0.22044
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.352
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	68532
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	296	0.2M calcium chloride, 0.1M HEPES-NaOH, 25%(w/v) polyethylene glycol 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K