4Q6M
Structural analysis of the apo-form of Helicobacter pylori Csd4, a D,L-carboxypeptidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0722 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.141, 66.549, 144.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.810 - 1.600 |
R-factor | 0.19491 |
Rwork | 0.194 |
R-free | 0.22044 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.352 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 68532 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 0.2M calcium chloride, 0.1M HEPES-NaOH, 25%(w/v) polyethylene glycol 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |