4Q5S

Thermus thermophilus RNA polymerase initially transcribing complex containing 6-mer RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.918
Spacegroup name	C 1 2 1
Unit cell lengths	187.089, 102.075, 297.263
Unit cell angles	90.00, 98.14, 90.00

Refinement procedure

Resolution	41.977 - 3.000
R-factor	0.2698
Rwork	0.269
R-free	0.29380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1563))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	107824
Completeness [%]	94.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	300	crystallization solution [100 mM Tris-HCl, pH 8.7, 200 mM KCl, 50 mM MgCl2, 10 mM Spermine tetra HCl and 10% PEG 4000], VAPOR DIFFUSION, HANGING DROP, temperature 300K