4Q4M
tRNA-Guanine Transglycosylase (TGT) in Complex with 6-Amino-4-phenyl-1,2-dihydro-1,3,5-triazin-2-one
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.89460 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.511, 63.887, 70.399 |
Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
Resolution | 26.837 - 1.621 |
R-factor | 0.1586 |
Rwork | 0.157 |
R-free | 0.18840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.006 |
RMSD bond angle | 1.068 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Number of reflections | 48799 | |
<I/σ(I)> | 21.5 | 2.8 |
Completeness [%] | 98.0 | 82.6 |
Redundancy | 3.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 100 mM MES, 10% DMSO, 13% PEG 8000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |