4Q4E
Crystal structure of E.coli aminopeptidase N in complex with actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-11-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.450, 120.450, 170.748 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.100 - 1.900 |
| R-factor | 0.14867 |
| Rwork | 0.147 |
| R-free | 0.18078 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.006 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.100 | 1.967 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 109675 | |
| Completeness [%] | 97.2 | 97.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.8M Sodium Malonate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
