4Q33
Crystal Structure of Inosine 5'-monophosphate Dehydrogenase from Clostridium perfringens Complexed with IMP and A110
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.121, 89.247, 99.185 |
| Unit cell angles | 70.82, 72.66, 79.30 |
Refinement procedure
| Resolution | 37.213 - 2.885 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q32 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.836 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.885 | 2.885 |
| Number of reflections | 59658 | |
| <I/σ(I)> | 7.6 | 1.5 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1 M HEPES, pH 7.0, 5% Tacsimate, pH 7.0, 10% PEG5000 MME, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
