4Q2Z
Fab fragment of HIV vaccine-elicited CD4bs-directed antibody, GE356, from a non-human primate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-18 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 48.243, 67.909, 69.980 |
Unit cell angles | 92.39, 94.13, 109.69 |
Refinement procedure
Resolution | 36.991 - 1.930 |
R-factor | 0.2343 |
Rwork | 0.232 |
R-free | 0.27240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ktd |
RMSD bond length | 0.005 |
RMSD bond angle | 1.119 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.620 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.549 | |
Number of reflections | 60025 | |
<I/σ(I)> | 19.2 | 1.1 |
Completeness [%] | 95.0 | 95.8 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M Tri-lithium citrate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |