4Q2C

Crystal structure of CRISPR-associated protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	104.268, 214.558, 102.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.781 - 2.500
R-factor	0.193
Rwork	0.191
R-free	0.22940
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.948
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	37133
Completeness [%]	93.3	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	2.2M Sodium Chloride, 0.1M Sodium phosphate monobasic monohydrate, 0.1M Potassium phosphate monobasic, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K