4Q0M

Crystal structure of Pyrococcus furiosus L-asparaginase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-03-28
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	116.380, 116.380, 153.941
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.893 - 2.226
R-factor	0.1994
Rwork	0.196
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4LX7
RMSD bond length	0.008
RMSD bond angle	1.054
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.170	2.330
High resolution limit [Å]	2.210	2.210
Rmerge	0.113	0.435
Number of reflections	19525
<I/σ(I)>	10.7	5.2
Completeness [%]	96.8	82.2
Redundancy	7.3	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	200mM Ammonium dihydrogen phosphate, 100mM Tris, 50% MPD, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K