4Q0M
Crystal structure of Pyrococcus furiosus L-asparaginase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-03-28 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 116.380, 116.380, 153.941 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.893 - 2.226 |
R-factor | 0.1994 |
Rwork | 0.196 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4LX7 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.054 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.170 | 2.330 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.113 | 0.435 |
Number of reflections | 19525 | |
<I/σ(I)> | 10.7 | 5.2 |
Completeness [%] | 96.8 | 82.2 |
Redundancy | 7.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 200mM Ammonium dihydrogen phosphate, 100mM Tris, 50% MPD, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |