4PZU
Crystal structure of a putative uncharacterize protein Rv3404c and likely sugar N-formyltransferase from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.080, 136.450, 99.970 |
Unit cell angles | 90.00, 103.74, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.1889 |
Rwork | 0.187 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nv1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.422 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.210 | |
High resolution limit [Å] | 2.100 | 9.620 | 2.150 |
Rmerge | 0.060 | 0.026 | 0.462 |
Number of reflections | 128170 | 1419 | 9325 |
<I/σ(I)> | 15.22 | 38.16 | 2.97 |
Completeness [%] | 99.8 | 96.5 | 99.9 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | MytuD.17389.a.A1.PW30616 at 19 mg/mL against JCSG+ screen condition D6, 0.2 M MgCl2, 0.1 M Tris pH 8.5, 20% PEG 8000 supplemented with 25% ethylene glycol, crystal tracking ID 220349d6, unique puck ID bdu3-7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |