4PZG

Crystal structure of human sorting nexin 10 (SNX10)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Detector technology	CCD
Collection date	2014-03-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	205.859, 205.859, 42.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.450 - 2.800
R-factor	0.1966
Rwork	0.194
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.970
Data scaling software	Aimless (0.1.29)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.450	49.450	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge	0.136	0.043	0.674
Total number of observations		8982	13007
Number of reflections	25144
<I/σ(I)>	10.5	27.2	2.2
Completeness [%]	98.7	99.5	94.6
Redundancy	6.5	10.5	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	4 M sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0, vapor diffusion, temperature 293K