4PZ4
High-resolution crystal structure of the human CD44 hyaluronan binding domain in new space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 70.271, 70.271, 286.458 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.860 - 1.600 |
R-factor | 0.1989 |
Rwork | 0.197 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.406 |
Data scaling software | SCALA |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 286.458 | 1.604 |
High resolution limit [Å] | 1.598 | 1.598 |
Rmerge | 0.063 | 0.490 |
Number of reflections | 56356 | |
<I/σ(I)> | 26.4 | 5.3 |
Completeness [%] | 99.3 | 80.4 |
Redundancy | 12.8 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7 | 277 | 25% PEG 3350, 100 mM NACl, 100 mM HEPES, (5% DMSO), pH 7.0, hanging drop, temperature 277K |