4PYZ

Crystal structure of the first two Ubl domains of Deubiquitylase USP7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97924
Spacegroup name	P 21 21 21
Unit cell lengths	68.328, 103.755, 116.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.710 - 2.840
R-factor	0.2452
Rwork	0.244
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ylm
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.880
High resolution limit [Å]	2.830	7.670	2.830
Rmerge	0.095	0.040	0.936
Number of reflections	19822
<I/σ(I)>	22.2
Completeness [%]	99.3	98.9	99.7
Redundancy	6.8	6.4	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	15% PEG 4000, 0.2 M NH4Ac, 0.1 M NaCitrate pH5.6, vapor diffusion hanging drop, temperature 291K