4PYG
Transglutaminase2 complexed with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-11-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 133.546, 216.218, 165.183 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.920 - 2.800 |
R-factor | 0.23985 |
Rwork | 0.236 |
R-free | 0.31904 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.631 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 55587 | |
Completeness [%] | 25.0 | 95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | 20mM Mes pH 6.8, 200mM sodium chloride, 20mM MgCl2, 6% PEG 3350, 5mM DTT, 24% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 297K |