4PXN
Structure of Zm ALDH7 in complex with NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-17 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 79.220, 162.490, 188.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.840 - 2.940 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.21710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jg7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.600 |
| High resolution limit [Å] | 2.940 |
| Number of reflections | 26136 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | MPD and 0.2 M ammonium phosphate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
