4PWN
Crystal structure of Active WNK1 kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.737, 64.226, 42.082 |
| Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
| Resolution | 35.180 - 1.840 |
| R-factor | 0.23114 |
| Rwork | 0.229 |
| R-free | 0.27363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3FPQ |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.180 |
| High resolution limit [Å] | 1.840 |
| Rmerge | 0.050 |
| Number of reflections | 19568 |
| <I/σ(I)> | 30.5 |
| Completeness [%] | 99.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 0.35M potassium phosphate, 19% PEG 3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
