4PWB
Phl p 4 I153V variant, a glucose oxidase, pressurized with Xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 117.640, 117.640, 202.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.407 - 1.900 |
| R-factor | 0.1611 |
| Rwork | 0.160 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tsh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (mr) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 123815 | |
| <I/σ(I)> | 19.32 | 1.27 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 7 mg/mL Protein in 20mM Tris + 70% Tacsimate,Crystals were soaked in 55% Tacsimate 20% Glycerol and pressurized with xenon before freezing, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
