4PRU
Crystal structure of dimethyllysine hen egg-white lysozyme in complex with sclx4 at 2.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.040, 30.280, 95.010 |
| Unit cell angles | 90.00, 96.62, 90.00 |
Refinement procedure
| Resolution | 39.410 - 2.200 |
| R-factor | 0.18288 |
| Rwork | 0.180 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pqr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.410 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.156 | 0.734 |
| Number of reflections | 13557 | |
| <I/σ(I)> | 7.9 | 3.7 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293.15 | 18 % PEG 8000 100 mM MAGNESIUM CHLORIDE, 50 mM SODIUM CHLORIDE, 50 mM SODIUM CACODYLATE PH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.15 K |
