4PRG
0072 PARTIAL AGONIST PPAR GAMMA COCRYSTAL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 92.720, 61.540, 118.810 |
| Unit cell angles | 90.00, 102.57, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.243 * |
| Rwork | 0.240 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 25.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.060 |
| Number of reflections | 118972 |
| Completeness [%] | 75.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 25 (mM) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | 200 (mM) | ||
| 5 | 1 | drop | EDTA | 1.5 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 15 (%) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) |
